Abstract
Author(s): Patel Hina B*and Patel Sejal K
The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective dual wavelength spectrophotometric method for the simultaneous determination of Gatifloxacin sesquihydrate(GAT) and Prednisolone acetate(PRD)in combined dosage form. The method was based on determination of Gatifloxacin sesquihydrate(GAT)at 346.0 nm and 269.6 nm give same absorbancebut PRD have zero absorbance at 346.0nm so, GAT directlymeasured at 346.0 nmand Prednisolone acetate(PRD)at the absorbance difference between 269.6 nm and 346 nm. The linearity was obtained in the concentration range of 2.5-40μg/ml for both drugs.LOQ values are found to be 0.51 μg/mL and 1.05 μg/mL for GAT and PRD in method. Percent label claim of the compounds were 99.77±1.16and 100.22±0.94in method for GAT and PRD respectively.The method was successfully applied to pharmaceutical dosage form because no interference from excipients was found. The suitability of these methods for the quantitative determination of Gatifloxacin sesquihydrate(GAT) and Prednisolone acetate(PRD)was proved by validation. The proposed methods were found to be simple and sensitive for the routine quality control application of Gatifloxacin sesquihydrate(GAT) and Prednisolone acetate(PRD)in pharmaceutical dosage form. The results of analysis have been validatedstatistically and by recovery studies.