ISSN: 2249-9504
CAS CODEN: IJPCDX

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IVABRADINE AND NEBIVOLOL IN TABLET DOSAGE FORMS

Abstract

Author(s): GK. Rajeswari1* , Siva Srikrishna2, T. Priyanka1 , P. Mounika1, G. Sadhana1 and B. Roshini1

A new, simple and sensitive Reverse phase performance liquid chromatography (RP-HPLC) method has been developed for the separation and quantification of Ivabradine (IVD) and Nebivolol (NBL) in tablet dosage form. The flow rate was maintained at 1.0ml/min and the eluent was monitored at 260nm. The retention time of IVD and NBL were 1.82min and 4.42 min respectively. The method was validated in terms of linearty, precision, accuracy, specificity and robustness. The method was linear and for precision studies; RSD for IVD and NBL were 0.02 and 0.04 respectively. The percentage recoveries for both drugs from their tablets were 100.80 and 99.76% respectively.

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